Abstract
Ayurveda utilizes herbo-mineral formulations with broad therapeutic applications. However, their mechanisms of action are not fully understood. Palashadi Varti, an Ayurvedic preparation, is traditionally used for vaginal disorders. Objective: This study evaluates its antimicrobial efficacy against various pathogens. Materials and Methods: Vaginal swab samples (n=30) were collected aseptically from patients at the OPD of Prasuti Tantra Evum Stree Roga, microbial isolates included Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus cereus from vaginal swabs and Klebsiella pneumoniae and Salmonella from the Department of Microbiology repository. Ethanolic extract of Palashadi Varti was prepared and tested at various concentrations (1000 mg/ml to 1.56 mg/ml) using the agar well diffusion method. The zone of inhibition (ZOI) was measured, and Colony Forming Units (CFU) before and after treatment were statistically analyzed. Results: The extract showed significant antimicrobial activity, particularly against Staphylococcus aureus and Bacillus cereus, with inhibition at concentrations as low as 31.2 mg/ml. Escherichia coli and Candida albicans were inhibited at 125 mg/ml, while Klebsiella pneumoniae required 250 mg/ml or higher. No activity was observed against Salmonella spp. In Group I (treatment group), the mean CFU reduced by 71.37% (Δ=103306.7 CFU/ml, p<0.001). Group II (control group) showed a reduction of 78.9% (Δ=90986.67 CFU/ml, p<0.001). Intergroup comparison indicated no statistically significant difference (p=0.308). Conclusion: Palashadi Varti exhibits promising antimicrobial activity, supporting its traditional use for vaginal infections.

Key words: Palashadi Varti, antimicrobial activity, vaginal infections, Ayurveda, ethanolic extract