Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation

Neelam Kumari Singh; Vaibhav Charde; Smriti Tandon; Hemant Rawat; Kishan Singh; Aakanksha Thakur; Satyendra Kumar; Mohd. Ehsan; T Maheswar; B.S Sharma; Shruti Khanduri; Ch. V. Narasimhaji; Sanjeev K Lale; Narayanam Srikanth; Rabinarayan Acharya

doi:10.5455/AAM.296098

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AAM. 2026; 15(3): 538-550

doi: 10.5455/AAM.296098

Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation

Neelam Kumari Singh, Vaibhav Charde, Smriti Tandon, Hemant Rawat, Kishan Singh, Aakanksha Thakur, Satyendra Kumar, Mohd. Ehsan, T Maheswar, B.s Sharma, Shruti Khanduri, Ch. V. Narasimhaji, Sanjeev K Lale, Narayanam Srikanth, Rabinarayan Acharya.

Abstract
Background: Panchkola Churna (PC) is a classical Ayurvedic formulation comprising Pippali, Pippalimool, Chavya, Chitraka, and Shunthi, used in the management of gastrointestinal disorders. Despite its therapeutic relevance, PC lacks analytical standardization and validated stability data.
Objectives: This study aimed to standardize PC by preparing three batches with authenticated raw materials from three different regions. The formulations were evaluated for pharmacognostical identity, physicochemical, microbial, and safety parameters, as well as marker-based high-performance thin-layer chromatography (HPTLC). Stability was evaluated according to the International Conference on Harmonization (ICH) guidelines.
Methods: Raw ingredients were authenticated and analyzed for their organoleptic, physicochemical, and microbiological properties in accordance with the Ayurvedic Pharmacopoeia of India (API). Finished formulations were subjected to quality control tests, powder flow analysis, HPTLC fingerprinting, and measurement of marker compounds such as piperine, piperlongumine, and 6-gingerol. An accelerated stability study was conducted to determine the shelf life.
Results: The QC parameters for the three PC batches included a total ash content of 6.03% w/w, an alcohol extractive value of 50.06% w/w, a water extractive value of 92.1% w/w, and a loss on drying of 6.96% w/w. Additionally, the acid-insoluble ash was 1.1% w/w, and the pH was 5.68. HPTLC analysis showed consistent Rf values for key markers across all batches. Quantitative tests identified piperine (387.85 µg/100 mg), piperlongumine (142.8 µg/100 mg), and 6-gingerol (25.3 µg/100 mg). Microbial contamination and heavy metal levels were within acceptable limits. The powder's flow properties demonstrated good compressibility and handling. Stability tests indicated a shelf-life of more than two years.
Conclusion: The study successfully established quality control parameters and validated the stability of PC. Marker-based HPTLC profiling, in conjunction with compliance to API and ICH standards, ensures its reproducibility, safety, and global acceptability.

Key words: Ayurveda, HPTLC, Panchkola Churna, Pharmaceutical Standardization, Quality control parameters, Shelf-life

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How to Cite this Article

Pubmed Style

Singh NK, Charde V, Tandon S, Rawat H, Singh K, Thakur A, Kumar S, Ehsan M, Maheswar T, Sharma B, Khanduri S, Narasimhaji CV, Lale SK, Srikanth N, Acharya R. Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation. AAM. 2026; 15(3): 538-550. doi:10.5455/AAM.296098

Web Style

Singh NK, Charde V, Tandon S, Rawat H, Singh K, Thakur A, Kumar S, Ehsan M, Maheswar T, Sharma B, Khanduri S, Narasimhaji CV, Lale SK, Srikanth N, Acharya R. Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation. https://www.aamjournal.in/?mno=296098 [Access: June 29, 2026]. doi:10.5455/AAM.296098

AMA (American Medical Association) Style

Singh NK, Charde V, Tandon S, Rawat H, Singh K, Thakur A, Kumar S, Ehsan M, Maheswar T, Sharma B, Khanduri S, Narasimhaji CV, Lale SK, Srikanth N, Acharya R. Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation. AAM. 2026; 15(3): 538-550. doi:10.5455/AAM.296098

Vancouver/ICMJE Style

Singh NK, Charde V, Tandon S, Rawat H, Singh K, Thakur A, Kumar S, Ehsan M, Maheswar T, Sharma B, Khanduri S, Narasimhaji CV, Lale SK, Srikanth N, Acharya R. Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation. AAM. (2026), [cited June 29, 2026]; 15(3): 538-550. doi:10.5455/AAM.296098

Harvard Style

Singh, N. K., Charde, . V., Tandon, . S., Rawat, . H., Singh, . K., Thakur, . A., Kumar, . S., Ehsan, . M., Maheswar, . T., Sharma, . B., Khanduri, . S., Narasimhaji, . C. V., Lale, . S. K., Srikanth, . N. & Acharya, . R. (2026) Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation. AAM, 15 (3), 538-550. doi:10.5455/AAM.296098

Turabian Style

Singh, Neelam Kumari, Vaibhav Charde, Smriti Tandon, Hemant Rawat, Kishan Singh, Aakanksha Thakur, Satyendra Kumar, Mohd. Ehsan, T Maheswar, B.s Sharma, Shruti Khanduri, Ch. V. Narasimhaji, Sanjeev K Lale, Narayanam Srikanth, and Rabinarayan Acharya. 2026. Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation. Annals of Ayurvedic Medicine, 15 (3), 538-550. doi:10.5455/AAM.296098

Chicago Style

Singh, Neelam Kumari, Vaibhav Charde, Smriti Tandon, Hemant Rawat, Kishan Singh, Aakanksha Thakur, Satyendra Kumar, Mohd. Ehsan, T Maheswar, B.s Sharma, Shruti Khanduri, Ch. V. Narasimhaji, Sanjeev K Lale, Narayanam Srikanth, and Rabinarayan Acharya. "Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation." Annals of Ayurvedic Medicine 15 (2026), 538-550. doi:10.5455/AAM.296098

MLA (The Modern Language Association) Style

Singh, Neelam Kumari, Vaibhav Charde, Smriti Tandon, Hemant Rawat, Kishan Singh, Aakanksha Thakur, Satyendra Kumar, Mohd. Ehsan, T Maheswar, B.s Sharma, Shruti Khanduri, Ch. V. Narasimhaji, Sanjeev K Lale, Narayanam Srikanth, and Rabinarayan Acharya. "Pharmaceutical Standardization and Stability Analysis of Panchkola Churna: A Polyherbal Ayurvedic Formulation." Annals of Ayurvedic Medicine 15.3 (2026), 538-550. Print. doi:10.5455/AAM.296098

APA (American Psychological Association) Style

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